Mouse IGFBP3 ELISA Kit (ab246528)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 41.08 pg/ml
- Range: 109.375 pg/ml - 7000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Culture Media
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IGFBP3 ELISA Kit
See all IGFBP3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 7.2% Inter-assay Sample n Mean SD CV% Serum 3 8.3% -
Sample type
Serum, Tissue Culture Media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
41.08 pg/ml -
Range
109.375 pg/ml - 7000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 104.5 99% - 109% Tissue Culture Media 101 88% - 104% Hep Plasma 107 102% - 110% EDTA Plasma 101 96% - 107% Cit plasma 96 90% - 102% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse IGFBP3 ELISA Kit (ab246528) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGFBP3 protein in cit plasma, edta plasma, hep plasma, serum, and tissue culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IGFBP3 with 41.08 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse IGFBP3 Capture Antibody 1 x 600µl 10X Mouse IGFBP3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Mouse IGFBP3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
Tissue specificity
Expressed by most tissues. Present in plasma. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Developmental stage
IGFBP3 levels are higher during extrauterine life and peak during puberty. -
Domain
The thyroglobulin type-1 domain mediates interaction with HN. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Acid stable subunit of the 140 K IGF complex
- Binding protein 29
- Binding protein 53
see all -
Database links
- Entrez Gene: 16009 Mouse
- SwissProt: P47878 Mouse
- Unigene: 29254 Mouse
Images
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Other - Mouse IGFBP3 ELISA Kit (ab246528)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse IGFBP3 Standard Curve Comparison.Standard Curve comparison between mouse IGFBP3 SimpleStep ELISA kit and traditional ELISA kit from leading competitor.
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Example of mouse IGFBP3 standard curve in Sample Diluent NS.The IGFBP3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IGFBP3 in mouse serum and plasma samples.The concentrations of IGFBP3 were measured in duplicates, interpolated from the IGFBP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 500, plasma (citrate) 1: 500, plasma (heparin) 1: 500, and plasma (EDTA) 1:500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP3 concentration was determined to be 1,461 ng/mL in serum, 1,546 ng/mL in plasma (citrate), 2,319 ng/mL in plasma (heparin), and 2,007 ng/ml in plasma (EDTA).
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Interpolated concentrations of native IGFBP3 in mouse liver tissue culture supernatant samples.The concentrations of IGFBP3 were measured in duplicates, interpolated from the IGFBP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: liver supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP3 concentration was determined to be 7,305 pg/mL in liver supernatant.
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Sandwich ELISA - Mouse IGFBP3 ELISA Kit (ab246528)To learn more about the advantages of recombinant antibodies see here.
