Mouse IGFBP3 Antibody Pair - BSA and Azide free (ab256673)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse
Overview
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Product name
Mouse IGFBP3 Antibody Pair - BSA and Azide free
See all IGFBP3 kits -
Assay type
ELISA set -
Range
109.375 pg/ml - 7000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
The Antibody Pair can be used to quantify Mouse IGFBP3. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab246528), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Mouse IGFBP3 Capture Antibody (unconjugated) 1 x 100µg Mouse IGFBP3 Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
Tissue specificity
Expressed by most tissues. Present in plasma. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Developmental stage
IGFBP3 levels are higher during extrauterine life and peak during puberty. -
Domain
The thyroglobulin type-1 domain mediates interaction with HN. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Acid stable subunit of the 140 K IGF complex
- Binding protein 29
- Binding protein 53
see all -
Database links
- Entrez Gene: 16009 Mouse
- SwissProt: P47878 Mouse
- Unigene: 29254 Mouse
Images
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Sandwich ELISA - Mouse IGFBP3 Antibody Pair - BSA and Azide free (ab256673)To learn more about the advantages of recombinant antibodies see here.
