Human ST2 ELISA Kit (IL1RL1) (ab254505)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 22.06 pg/ml
- Range: 109.38 pg/ml - 7000 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ST2 ELISA Kit (IL1RL1)
See all ST2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.2% Inter-assay Sample n Mean SD CV% Serum 3 4.4% -
Sample type
Cell culture supernatant, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
22.06 pg/ml -
Range
109.38 pg/ml - 7000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 114 108% - 118% Serum 108 98% - 118% Cell culture media 87 81% - 94% Hep Plasma 89 81% - 96% EDTA Plasma 107 82% - 118% Cit plasma 98 84% - 108% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human ST2 ELISA Kit (IL1RL1) (ab254505) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ST2 (IL1RL1) protein in cell culture supernatant, cit plasma, hep plasma, serum, edta plasma, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ST2 (IL1RL1) with 22.06 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
ST2, also known as or Interleukin 1 receptor-like 1 (IL-1RL1), is a protein encoded by the IL1RL1 gene. ST2 gene is a member of the interleukin 1 receptor family. ST2, interleukin 1 receptor, type II and interleukin 1 receptor like 2 form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. Studies of the similar gene in mouse reported protein induction by pro-inflammatory stimuli with involvement in the function of helper T cells. ST2 is necessary for endotoxin tolerance by inhibiting TLR responses inducing the Th2 response. Human ST2 shares 68%, 64%, 66%, and 96% sequence homology with mouse, rat, bovine, and monkey, respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human ST2 (IL1RL1) Capture Antibody 1 x 600µl 10X Human ST2 (IL1RL1) Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human ST2 (IL1RL1) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function. -
Tissue specificity
Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle. -
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- DER-4
- DER4
- FIT 1
see all -
Database links
- Entrez Gene: 9173 Human
- Omim: 601203 Human
- SwissProt: Q01638 Human
- Unigene: 66 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human ST2 standard curve in Sample Diluent NS.The ST2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native ST2 in human serum and plasma samples.The concentrations of ST2 were measured in duplicates, interpolated from the ST2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50% and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ST2 concentration was determined to be 13.86 ng/mL in serum, 9.81 ng/mL in plasma (citrate), 12.62 ng/mL in plasma (EDTA), and 9.06 ng/mL in plasma (Heparin).
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Interpolated concentrations of native ST2 in human cell culture supernatant samples.The concentrations of ST2 were measured in duplicates, interpolated from the ST2 standard curves and corrected for sample dilution. Undiluted samples are as follows: HUVEC cell culture supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ST2 concentration was determined to be 68.42 ng/mL in HUVEC cell culture supernatant.
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10% Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ST2 concentration was determined to be 10.13 ng/mL with a range of 5.18 – 15.85 ng/mL.
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10% Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ST2 concentration was determined to be 12.42 ng/mL with a range of 7.16 – 20.19 ng/mL.
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Sandwich ELISA - Human ST2 ELISA Kit (IL1RL1) (ab254505)To learn more about the advantages of recombinant antibodies see here.
