Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human GCLC ELISA Kit (ab233632)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 81 pg/ml
  • Range: 781.3 pg/ml - 50000 pg/ml
  • Sample type: Cell culture extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human GCLC ELISA Kit
    See all GCLC kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 8 7%

  • Sample type

    Cell culture extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    81 pg/ml

  • Range

    781.3 pg/ml - 50000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 95 % - %

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human GCLC ELISA Kit (ab233632) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GCLC protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human GCLC with 81 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Human GCLC ELISA Kit (ab233632)
    Other - Human GCLC ELISA Kit (ab233632)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human GCLC standard curve in 1X Cell Extraction Buffer PTR.
    Example of human GCLC standard curve in 1X Cell Extraction Buffer PTR.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native GCLC in MCF-7 cell extract samples based on a 1,000 ?g/mL extract load.
    Interpolated concentrations of native GCLC in MCF-7 cell extract samples based on a 1,000 ?g/mL extract load.

    The concentrations of GCLC were measured in duplicate and interpolated from the GCLC standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GCLC concentration was determined to be 3,074 pg/mL.

  • Sandwich ELISA - Human GCLC ELISA Kit (ab233632)
    Sandwich ELISA - Human GCLC ELISA Kit (ab233632)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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