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Signal Transduction Metabolism Mitochondrial

Human GCLC Antibody Pair - BSA and Azide free (ab241899)

Human GCLC Antibody Pair - BSA and Azide free (ab241899)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

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Overview

  • Product name

    Human GCLC Antibody Pair - BSA and Azide free
    See all GCLC kits
  • Assay type

    ELISA set
  • Range

    78.125 pg/ml - 5000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Antibody Pair can be used to quantify Human GCLC. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab233632), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human GCLC Capture Antibody (unconjugated) 1 x 100µg
    Human GCLC Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cell Biology
    • Other Antibodies
    • Other Antibodies
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
  • Pathway

    Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
  • Involvement in disease

    Defects in GCLC are the cause of hemolytic anemia (HAGGSD) [MIM:230450].
  • Sequence similarities

    Belongs to the glutamate--cysteine ligase type 3 family.
  • Target information above from: UniProt accession P48506 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • EC 6.3.2.2
    • Gamma ECS
    • Gamma glutamylcysteine synthetase
    • Gamma-ECS
    • Gamma-glutamylcysteine synthetase
    • GCL
    • Gclc
    • GCS
    • GCS heavy chain
    • GLCL
    • GLCLC
    • Glutamate cysteine ligase catalytic subunit
    • Glutamate--cysteine ligase catalytic subunit
    • GSH1_HUMAN
    see all
  • Database links

    • Entrez Gene: 2729 Human
    • Omim: 606857 Human
    • SwissProt: P48506 Human
    • Unigene: 654465 Human

    Images

    • Sandwich ELISA - Human GCLC Antibody Pair - BSA and Azide free (ab241899)
      Sandwich ELISA - Human GCLC Antibody Pair - BSA and Azide free (ab241899)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Alternative products to Human GCLC Antibody Pair - BSA and Azide free (ab241899)

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      Applications:

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      Human GCLC Matched Antibody Pair Kit (ab222267)

      Applications:

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