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Signal Transduction Metabolism Mitochondrial

Human GCLC Matched Antibody Pair Kit (ab222267)

Price and availability

301 536 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Human GCLC Matched Antibody Pair Kit (ab222267)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Human
  • Range: 78.125 pg/ml - 5000 pg/ml

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Overview

  • Product name

    Human GCLC Matched Antibody Pair Kit
    See all GCLC kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Sensitivity

    2.7 pg/ml
  • Range

    78.125 pg/ml - 5000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human GCLC Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human GCLC.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab233632. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 5 x 96 tests
    Human GCLC Capture Antibody 2 x 50µg 1 x 50µg
    Human GCLC Detector Antibody 2 x 12.5µg 1 x 12.5µg
    Human GCLC Lyophilized Protein 2 vials 1 vial
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cell Biology
    • Other Antibodies
    • Other Antibodies
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
  • Pathway

    Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
  • Involvement in disease

    Defects in GCLC are the cause of hemolytic anemia (HAGGSD) [MIM:230450].
  • Sequence similarities

    Belongs to the glutamate--cysteine ligase type 3 family.
  • Target information above from: UniProt accession P48506 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • EC 6.3.2.2
    • Gamma ECS
    • Gamma glutamylcysteine synthetase
    • Gamma-ECS
    • Gamma-glutamylcysteine synthetase
    • GCL
    • Gclc
    • GCS
    • GCS heavy chain
    • GLCL
    • GLCLC
    • Glutamate cysteine ligase catalytic subunit
    • Glutamate--cysteine ligase catalytic subunit
    • GSH1_HUMAN
    see all
  • Database links

    • Entrez Gene: 2729 Human
    • Omim: 606857 Human
    • SwissProt: P48506 Human
    • Unigene: 654465 Human

    Images

    • Human GCLC standard curve
      Human GCLC standard curve

      Standard calibration curve. Background subtracted values are graphed.

    • Sandwich ELISA - Human GCLC Matched Antibody Pair Kit (ab222267)
      Sandwich ELISA - Human GCLC Matched Antibody Pair Kit (ab222267)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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