Human ERK1 Matched Antibody Pair Kit (ab219531)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 125 pg/ml - 8000 pg/ml
Overview
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Product name
Human ERK1 Matched Antibody Pair Kit
See all ERK1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
49 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human ERK1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human ERK1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human ERK1 Capture Antibody 2 x 50µg 1 x 50µg Human ERK1 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human ERK1 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204. - Information by UniProt
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Alternative names
- ERK 1
- ERK-1
- ERK1
see all -
Database links
- Entrez Gene: 5595 Human
- Omim: 601795 Human
- SwissProt: P27361 Human
- Unigene: 861 Human
Images
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Human ERK1 standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human ERK1 Matched Antibody Pair Kit (ab219531)To learn more about the advantages of recombinant antibodies see here.
