Human CD30-L ELISA Kit (TNFSF8) (ab252362)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.06 ng/ml
- Range: 0.23 ng/ml - 15 ng/ml
- Sample type: Cell culture extracts, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD30-L ELISA Kit (TNFSF8)
See all CD30-L kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 3.7% Inter-assay Sample n Mean SD CV% Extract 3 4.98% -
Sample type
Serum, Cell culture extracts, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.06 ng/ml -
Range
0.23 ng/ml - 15 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 97 89% - 102% Cell culture extracts 99 95% - 101% Tissue Extracts 90 90% - 90% EDTA Plasma 95 91% - 98% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD30-L ELISA Kit (TNFSF8) (ab252362) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD30-L (TNFSF8) protein in edta plasma, serum, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD30-L (TNFSF8) with 0.06 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CD30-L (TNFSF8) Capture Antibody 1 x 600µl 10X Human CD30-L (TNFSF8) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human CD30-L (TNFSF8) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD153
- CD153 antigen
- CD30 antigen ligand
see all -
Database links
- Entrez Gene: 944 Human
- Omim: 603875 Human
- SwissProt: P32971 Human
- Unigene: 654445 Human
Images
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Other - Human CD30-L ELISA Kit (ab252362)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CD30-L standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.The CD30-L standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human CD30-L standard curve in Sample Diluent NS.The CD30-L standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked CD30-L in human Ramos cell extract and thymus extract based on a 250 µg/mL extract load.The concentrations of CD30-L were measured in duplicate and interpolated from the CD30-L standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of spike CD30-L in human serum and plasma (EDTA) samples.The concentrations of CD30-L were measured in duplicates, interpolated from the CD30-L standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50% and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Serum from eight individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD30-L concentration was determined to be 1.3 ng/mL with a range of 0.83 – 1.6 ng/mL.
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Sandwich ELISA - Human CD30-L ELISA Kit (TNFSF8) (ab252362)To learn more about the advantages of recombinant antibodies see here.
