Human 14-3-3 tau ELISA Kit (ab246543)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6.1 pg/ml
- Range: 23.4 pg/ml - 1500 pg/ml
- Sample type: Cell culture extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human 14-3-3 tau ELISA Kit
See all 14-3-3 Tau kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% extract 8 3.3% Inter-assay Sample n Mean SD CV% extract 3 7% -
Sample type
Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
6.1 pg/ml -
Range
23.4 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 94 80% - 107% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human 14-3-3 tau ELISA Kit (ab246543) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of 37694 tau protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human 37694 tau with 6.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
14-3-3 Tau, also known as 14-3-3- theta, is a 245- amino acid containing adapter protein encoded by the gene YWHAQ. 14-3-3 tau belongs to the 14-3-3 family of proteins that are involved in a broad range of cell signaling pathways by binding to numerous other proteins, generally by recognition of either a phosphoserine or phosphothreonine motif. 14-3-3 tau is highly conserved across species and has been shown to be involved in cellular processes including metabolism, protein trafficking, signal transduction, and apoptosis.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human 14-3-3 tau Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human 14-3-3 tau Capture Antibody (Lyophilized) 1 x 600µl Human 14-3-3 tau Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1. -
Tissue specificity
Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement. -
Sequence similarities
Belongs to the 14-3-3 family. -
Post-translational
modificationsSer-232 is probably phosphorylated by CK1. -
Cellular localization
Cytoplasm. In neurons, axonally transported to the nerve terminals. - Information by UniProt
-
Alternative names
- 14 3 3 protein T cell
- 14 3 3 protein tau
- 14 3 3 protein theta
see all -
Database links
- Entrez Gene: 10971 Human
- Omim: 609009 Human
- SwissProt: P27348 Human
- Unigene: 74405 Human
Images
-
Other - Human 14-3-3 tau ELISA Kit (ab246543)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human 14-3-3 tau standard curve in 1X Cell Extraction Buffer PTR.The 14-3-3 tau standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native 14-3-3 tau in human serum, plasma and cell culture supernatant samples.The concentrations of 14-3-3 tau were measured in duplicates, interpolated from the 14-3-3 tau standard curves and corrected for sample dilution. Undiluted samples are as follows: A549 cell extract 0.6 µg/mL, Raji cell extract 0.3 µg/mL, and NIH/3T3 cell extract 0.6 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
-
Sandwich ELISA - Human 14-3-3 tau ELISA Kit (ab246543)To learn more about the advantages of recombinant antibodies see here.
