Human 14-3-3 tau Antibody Pair - BSA and Azide free (ab256688)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human 14-3-3 tau Antibody Pair - BSA and Azide free
See all 14-3-3 Tau kits -
Assay type
ELISA set -
Range
23.4 pg/ml - 1500 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human 14-3-3 tau. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab246543), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human 14-3-3 tau Capture Antibody (unconjugated) 1 x 100µg Human 14-3-3 tau Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1. -
Tissue specificity
Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement. -
Sequence similarities
Belongs to the 14-3-3 family. -
Post-translational
modificationsSer-232 is probably phosphorylated by CK1. -
Cellular localization
Cytoplasm. In neurons, axonally transported to the nerve terminals. - Information by UniProt
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Alternative names
- 14 3 3 protein T cell
- 14 3 3 protein tau
- 14 3 3 protein theta
see all -
Database links
- Entrez Gene: 10971 Human
- Omim: 609009 Human
- SwissProt: P27348 Human
- Unigene: 74405 Human
Images
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Sandwich ELISA - Human 14-3-3 tau Antibody Pair - BSA and Azide free (ab256688)To learn more about the advantages of recombinant antibodies see here.
