Human SFN ELISA Kit (ab245711)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 115 pg/ml
- Range: 0.156 ng/ml - 10 ng/ml
- Sample type: Cell culture extracts, EDTA Plasma, Saliva, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human SFN ELISA Kit
See all 14-3-3 sigma/SFN kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% saliva 6 7.4% Inter-assay Sample n Mean SD CV% saliva 3 9.9% -
Sample type
Saliva, Serum, Cell culture extracts, Tissue Extracts, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
115 pg/ml -
Range
0.156 ng/ml - 10 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 99 89% - 108% Serum 92 90% - 96% Cell culture extracts 91 76% - 100% Tissue Extracts 98 96% - 99% EDTA Plasma 84 82% - 89% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human SFN ELISA Kit (ab245711) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SFN protein in edta plasma, saliva, serum, tissue extracts, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SFN with 115 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human SFN Capture Antibody 1 x 600µl 10X Human SFN Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human SFN Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
p53-regulated inhibitor of G2/M progression. -
Tissue specificity
Present mainly in tissues enriched in stratified squamous keratinizing epithelium. -
Sequence similarities
Belongs to the 14-3-3 family. -
Post-translational
modificationsUbiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation. -
Cellular localization
Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway. - Information by UniProt
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Alternative names
- 14 3 3 protein sigma
- 14-3-3 protein sigma
- 1433S_HUMAN
see all -
Database links
- Entrez Gene: 2810 Human
- Omim: 601290 Human
- SwissProt: P31947 Human
- Unigene: 523718 Human
Images
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Other - Human SFN ELISA Kit (ab245711)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human SFN standard curve in Sample Diluent NS.The SFN standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Example of human SFN standard curve in 1X Cell Extraction Buffer PTRThe SFN standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native SFN in human skin, PC3, and A431 cell extracts.The concentrations of SFN were measured in duplicate and interpolated from the SFN standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). -
Interpolated concentrations of native SFN in male human saliva samples.The concentrations of SFN were measured in duplicates, interpolated from the SFN standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SFN concentration was determined to be 120.5 ng/mL in neat saliva. -
Sandwich ELISA - Human SFN ELISA Kit (ab245711)To learn more about the advantages of recombinant antibodies see here.
