Antibodies, Proteins, Kits and Reagents for Life Science

HRP Anti-CD46 antibody [EPR4014] (ab196863)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
HRP Rabbit monoclonal [EPR4014] to CD46
Suitable for: WB
Knockout validated
Reacts with: Human
Conjugation: HRP

Product name

HRP Anti-CD46 antibody [EPR4014]
See all CD46 primary antibodies
Description

HRP Rabbit monoclonal [EPR4014] to CD46
Host species

Rabbit
Conjugation

HRP
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa whole cell lysate.
General notes
Alternative versions available:
Anti-CD46 antibody [EPR4014] (ab108307) - Knockout validated
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4014
Isotype

IgG
Research areas

Western blot - HRP Anti-CD46 antibody [EPR4014] (ab196863)

All lanes : Anti-NDUFS3 antibody [EPR12782] - C-terminal (Alexa Fluor® 647) (ab196864) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CD46 knockout HAP1 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 44 kDa

Exposure time: 20 minutes

ab196863 was shown to recognize CD46 in wild-type HAP1 cells as signal was lost at the expected MW in CD46 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CD46 knockout samples were subjected to SDS-PAGE. Ab196863 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor^® 680) loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
Western blot - HRP Anti-CD46 antibody [EPR4014] (ab196863)

HRP Anti-CD46 antibody [EPR4014] (ab196863) at 1/1000 dilution + HeLa whole cell lysate (ab150035) at 10 µg

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 44 kDa
Observed band size: 52 kDa
why is the actual band size different from the predicted?

Exposure time: 20 minutes

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196863 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
HRP Anti-CD46 antibody [EPR4014] (ab196863)

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