Antibodies, Proteins, Kits and Reagents for Life Science

361 843 ₸ Product size

100 µl 361 843 ₸ 10 µl 144 067 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4014] to CD46
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-CD46 antibody [EPR4014]
See all CD46 primary antibodies
Description

Rabbit monoclonal [EPR4014] to CD46
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Wild-type HAP1 cell lysate. MOLT-4, Jurkat, HeLa and K562 cell lysates. IHC-P: Human thyroid gland carcinoma, colonic adenocarcinoma, breast carcinoma, kidney, breast, uterus, placenta and tonsil tissue.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4014
Isotype

IgG
Research areas

Western blot - Anti-CD46 antibody [EPR4014] (ab108307)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CD46 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108307 observed at 50-70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab108307 was shown to specifically react with when CD46 knockout samples were used. Wild-type and CD46 knockout samples were subjected to SDS-PAGE. ab108307 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)

ab108307, at 1/500 dilution, staining CD46 in paraffin-embedded human tonsil tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)

ab108307, at 1/500 dilution, staining CD46 in paraffin-embedded human kidney tissue

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Western blot - Anti-CD46 antibody [EPR4014] (ab108307)

All lanes : Anti-CD46 antibody [EPR4014] (ab108307) at 1/1000 dilution

Lane 1 : MOLT-4 (Human lymphoblastic leukemia cell line) cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution

Predicted band size: 44 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)

ab108307 showing positive staining in human normal placenta tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)

ab108307 showing positive staining in human normal uterus tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)

ab108307 showing positive staining in human thyroid gland carcinoma tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)

ab108307 showing positive staining in human colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)

ab108307 showing positive staining in human breast carcinoma tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (ab108307)

ab108307 showing positive staining in human normal breast tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Anti-CD46 antibody [EPR4014] (ab108307)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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