Antibodies, Proteins, Kits and Reagents for Life Science

341 740 ₸ Product size

100 µl 341 740 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6554] to SUN1
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-SUN1 antibody [EPR6554]
See all SUN1 primary antibodies
Description

Rabbit monoclonal [EPR6554] to SUN1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human SUN1 aa 100-200.
Positive control
- IHC-P: Human ovary carcinoma and human breast tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6554
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN1 antibody [EPR6554] (ab124770)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling SUN1 with purified ab124770 at 1/200 dilution (0.698 µg/ml). Perform heat mediated antigen retrieval using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-SUN1 antibody [EPR6554] (ab124770)

All lanes : Anti-SUN1 antibody [EPR6554] (ab124770) at 1/5000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 90 kDa
Observed band size: 100,90 kDa
why is the actual band size different from the predicted?

The bands observed are consistent with what has been described in PMID: 16648470
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN1 antibody [EPR6554] (ab124770)

Unpurified ab124770, at 1/100 dilution, staining SUN1 in paraffin-embedded Human ovary carcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-SUN1 antibody [EPR6554] (ab124770)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com