Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-TPK1 antibody [EPR10918] (ab170863)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10918] to TPK1
Suitable for: IHC-P, WB, IP
Reacts with: Human

Product name

Anti-TPK1 antibody [EPR10918]
See all TPK1 primary antibodies
Description

Rabbit monoclonal [EPR10918] to TPK1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human TPK1 aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9H3S4
Positive control
- SH-SY5Y, JAR, Human fetal kidney, Human fetal brain and Human fetal lung lysates. Heart, kidney and tonsi tissue. Immunoprecipitation pellet from fetal brain lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10918
Isotype

IgG

Western blot - Anti-TPK1 antibody [EPR10918] (ab170863)

All lanes : Anti-TPK1 antibody [EPR10918] (ab170863) at 1/1000 dilution

Lane 1 : SH-SY5Y lysate
Lane 2 : JAR lysate
Lane 3 : Fetal kidney lysate
Lane 4 : Fetal brain lysate
Lane 5 : Fetal lung lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 27 kDa
Immunoprecipitation - Anti-TPK1 antibody [EPR10918] (ab170863)

Western blot analysis on immunoprecipitation pellet from (Lane 1) fetal brain lysate or (Lane 2) 1X PBS (negative control) using ab170863 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPK1 antibody [EPR10918] (ab170863)

Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded Human tonsil tissue labeling TPK1 with ab170863 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPK1 antibody [EPR10918] (ab170863)

Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded Human kidney tissue labeling TPK1 with ab170863 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPK1 antibody [EPR10918] (ab170863)

Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded Human heart tissue labeling TPK1 with ab170863 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-TPK1 antibody [EPR10918] (ab170863)

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