PE Anti-CD133 antibody [EPR20980-104] (ab252128)
![PE Anti-CD133 antibody [EPR20980-104] (ab252128)](https://www.abcam.com/ps/products/252/ab252128/Images/ab252128-1-Rabbit-monoclonal-EPR20980104-to-CD133-RPE--NCCIT-cells-flow-cytometry.jpg "PE Anti-CD133 antibody [EPR20980-104] (ab252128)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR20980-104] to CD133
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-CD133 antibody [EPR20980-104]
See all CD133 primary antibodies -
Description
PE Rabbit monoclonal [EPR20980-104] to CD133 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cytometry: NCCIT (Positive control). HEK293 (Negative control).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20980-104 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - PE Anti-CD133 antibody [EPR20980-104] (ab252128)
Overlay histogram showing left NCCIT positive cells and right negative HEK293 cells stained with ab252128 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab252128) (1x 106 in 100µl at 0.5 µg/ml (1/1000)) for 30 min on ice.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
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![PE Anti-CD133 antibody [EPR20980-104] (ab252128)](https://www.abcam.com/ps/products/252/ab252128/Images/ab252128-2-benefits-of-recombinant-antibodies.png)
PE Anti-CD133 antibody [EPR20980-104] (ab252128)
