Biotin Anti-Leptin antibody (ab271278)
Key features and details
- Biotin Rabbit polyclonal to Leptin
- Suitable for: WB, ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Leptin antibody
See all Leptin primary antibodies -
Description
Biotin Rabbit polyclonal to Leptin -
Host species
Rabbit -
Conjugation
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentWB Recombinant fragment
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Immunogen
Recombinant full length protein corresponding to Human Leptin aa 1 to the C-terminus. Mature chain. E. coli derived.
Database link: P41159 -
Positive control
- WB: Recombinant human Leptin.
Properties
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Form
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Biotin Anti-Leptin antibody (ab271278)
Lane 1: Marker.
Lane 2: Recombinant hLeptin at 250ng/lane.
Lane 3: Recombinant hLeptin at 125ng/lane.
Lane 4: Recombinant hLeptin at 62.5ng/lane.
Lane 5: Recombinant hLeptin at 31.25ng/lane.
Lane 6: Recombinant hLeptin at 15.625ng/lane.
Lane 7: Recombinant hLeptin at 7.8ng/lane.
Lane 8: Recombinant hLeptin at 3.9ng/lane.
Lane 9: Recombinant hLeptin at 1.95ng/lane.
Lane 10: Recombinant hLeptin at 0.975ng/lane.
Lane 11: Recombinant hLeptin at 0.4875ng/lane.
Lane 12: Recombinant hLeptin at 0.24ng/lane. -
ELISA - Biotin Anti-Leptin antibody (ab271278)Human Leptin was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271278. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLeptin protein.