Anti-Glucagon antibody - N-terminal (ab189279)
Key features and details
- Rabbit polyclonal to Glucagon - N-terminal
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Glucagon antibody - N-terminal
See all Glucagon primary antibodies -
Description
Rabbit polyclonal to Glucagon - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Glucagon aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P01275 -
Positive control
- Human pancreas, islets of Langerhans; Human hepatocarcinoma tissue; HepG2 cells; Mouse bladder tissue lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody - N-terminal (ab189279)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas, islets of Langerhans labeling Glucagon with ab189279 at 10 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody - N-terminal (ab189279)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue labeling Glucagon with ab189279 at 10 µg/ml, followed by peroxidase-conjugated secondary antibody and DAB staining.
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Flow Cytometry - Anti-Glucagon antibody - N-terminal (ab189279)Flow cytometric analysis of HepG2 cells labeling Glucagon with ab189279 at 1/10 dilution (bottom histogram) compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot - Anti-Glucagon antibody - N-terminal (ab189279)Anti-Glucagon antibody - N-terminal (ab189279) at 1/1000 dilution + Mouse bladder tissue lysate at 35 µg
Predicted band size: 21 kDa
