Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glucagon antibody [EP3070] (ab92517)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3070] to Glucagon
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Glucagon antibody [EP3070]
    See all Glucagon primary antibodies

  • Description

    Rabbit monoclonal [EP3070] to Glucagon

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Glucagon aa 50-150 (N terminal). The exact sequence is proprietary.

  • Positive control

    • IHC-P: Murine, human, and rat pancreatic tissue. IHC-Fr: Mouse pancreas tissue. WB: Capan-1 cell lysate, Mouse and rat pancreas tissue lysate

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517) Image from Oelze M et al., PLoS One. 2014;9(11):e112394. Fig 1.; doi: 10.1371/journal.pone.0112394. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Glucagon measurements in controls and diabetic rats 8 weeks after STZ injection and 7 weeks of SGLT2i (low and high dose) treatment. Representative immunohistochemical stainings of pancreatic tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat pancreas tissue sections labeling Glucagon with Purified ab92517 at 1:8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0)Goat Anti-Rabbit IgG H&L (HRP) ab97051was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
  • Western blot - Anti-Glucagon antibody [EP3070] (ab92517)
    Western blot - Anti-Glucagon antibody [EP3070] (ab92517)
    Anti-Glucagon antibody [EP3070] (ab92517) at 0.3 µg/ml (purified) + Capan-1 (Human pancreas adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse pancreas tissue sections labeling Glucagon with Purified ab92517 at 1:8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0)Goat Anti-Rabbit IgG H&L (HRP) ab97051was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
  • Western blot - Anti-Glucagon antibody [EP3070] (ab92517)
    Western blot - Anti-Glucagon antibody [EP3070] (ab92517)
    Anti-Glucagon antibody [EP3070] (ab92517) at 0.3 µg/ml (purified) + Mouse pancreas lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreas tissue sections labeling Glucagon with Purified ab92517 at 1:8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0)Goat Anti-Rabbit IgG H&L (HRP) ab97051was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
  • Western blot - Anti-Glucagon antibody [EP3070] (ab92517)
    Western blot - Anti-Glucagon antibody [EP3070] (ab92517)
    Anti-Glucagon antibody [EP3070] (ab92517) at 0.3 µg/ml (purified) + Rat pancreas lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517) Image is courtesy of an anonymous AbReview.

    Immunohistochemical analysis of PFA-fixed paraffin-embedded murine pancreatic tissue sections, labelling glucagon with unpurified ab92517 at a dilution of 1/2500 incubated for 18 hours at 4°C. Antigen retrival was performed through heat-mediated 10mM sodium citrate pH 6.0 incubation. Blocking was done with 5% serum for 1 hour at 25°C. The secondary was a donkey anti-rabbit polyclonal Alexa Fluor® 488 conjugate at 1/400. Counterstaining is with DAPI (blue) against nuclear DNA.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)

    Unpurified ab92517, at 1/1000 dilution, staining Glucagon in paraffin embedded Human pancreas tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Glucagon antibody [EP3070] (ab92517)
    Anti-Glucagon antibody [EP3070] (ab92517)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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