Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-U2AF65 antibody [EPR17046] - BSA and Azide free (ab251237)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17046] to U2AF65 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-U2AF65 antibody [EPR17046] - BSA and Azide free
    See all U2AF65 primary antibodies

  • Description

    Rabbit monoclonal [EPR17046] to U2AF65 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251237 is the carrier-free version of ab197031. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

     

    ab251237 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-U2AF65 antibody [EPR17046] - BSA and Azide free (ab251237)
    Western blot - Anti-U2AF65 antibody [EPR17046] - BSA and Azide free (ab251237)
    All lanes : Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031) at 1/20000 dilution

    Lane 1 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa


    Exposure time: 1 minute


    This data was developed using ab197031, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-U2AF65 antibody [EPR17046] - BSA and Azide free (ab251237)
    Immunocytochemistry - Anti-U2AF65 antibody [EPR17046] - BSA and Azide free (ab251237)

    This data was developed using ab197031, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling U2AF65 with Purified ab197031 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody [EPR17046] - BSA and Azide free (ab251237)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody [EPR17046] - BSA and Azide free (ab251237)

    This data was developed using ab197031, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling U2AF65 with ab197031 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human endometrial adenocarcinoma tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-U2AF65 antibody [EPR17046] - BSA and Azide free (ab251237)
    Western blot - Anti-U2AF65 antibody [EPR17046] - BSA and Azide free (ab251237)
    All lanes : Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031) at 1/2000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa


    Exposure time: 1 minute


    This data was developed using ab197031, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-U2AF65 antibody [EPR17046] - BSA and Azide free (ab251237)
    Western blot - Anti-U2AF65 antibody [EPR17046] - BSA and Azide free (ab251237)
    Anti-U2AF65 antibody [EPR17046] - C-terminal (ab197031) at 1/2000 dilution + Mouse brain tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa


    Exposure time: 3 minutes


    This data was developed using ab197031, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody [EPR17046] - BSA and Azide free (ab251237)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody [EPR17046] - BSA and Azide free (ab251237)
    This data was developed using ab197031, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling U2AF65 with ab197031 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Mouse liver tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody [EPR17046] - BSA and Azide free (ab251237)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody [EPR17046] - BSA and Azide free (ab251237)
    This data was developed using ab197031, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling U2AF65 with ab197031 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on rat cerebral cortex tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-U2AF65 antibody [EPR17046] - BSA and Azide free (ab251237)
    Anti-U2AF65 antibody [EPR17046] - BSA and Azide free (ab251237)

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