Anti-hnRNP R antibody (ab30930)
Key features and details
- Rabbit polyclonal to hnRNP R
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-hnRNP R antibody
See all hnRNP R primary antibodies -
Description
Rabbit polyclonal to hnRNP R -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Zebrafish
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human hnRNP R.
Read Abcam's proprietary immunogen policy (Peptide available as ab31802.) -
Positive control
- HeLa (Human epithelial carcinoma cell line) Nuclear Lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Testis (Mouse) Tissue Lysate - normal tissue Ovary (Mouse) Tissue Lysate - normal tissue PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab30930 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 1 µg/ml. WB 1/500. Detects a band of approximately 68, 73, 76 kDa (predicted molecular weight: 71 kDa). Target
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Function
Component of ribonucleosomes, which are complexes of at least 20 other different heterogenious nuclear ribonucleoproteins (hnRNP). hnRNP play an important role in processing of precursor mRNA in the nucleus. -
Sequence similarities
Contains 3 RRM (RNA recognition motif) domains. -
Cellular localization
Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 10236 Human
- Entrez Gene: 74326 Mouse
- Entrez Gene: 319110 Rat
- Entrez Gene: 406735 Zebrafish
- Omim: 607201 Human
- SwissProt: O43390 Human
- Unigene: 373763 Human
- Unigene: 31051 Mouse
see all -
Alternative names
- FLJ25714 antibody
- Heterogeneous nuclear ribonucleoprotein R antibody
- hnRNP R antibody
see all
Images
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Western blot - Anti-hnRNP R antibody (ab30930)Anti-hnRNP R antibody (ab30930) at 1 µg/ml + HeLa Nuclear lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 71 kDaBlocking buffer: 2% BSA
Gel type: MOPS
Observed band size: 73, 77 and 80 kDa
Exposure Time: 1 minute
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Western blot - Anti-hnRNP R antibody (ab30930)All lanes : Anti-hnRNP R antibody (ab30930) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 4 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 73,77,80 kDa why is the actual band size different from the predicted?
The three bands observed are thought to be differentially processed forms of hnRNP R. -
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP R antibody (ab30930)ICC/IF image of ab30930 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab30930, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Protocols
Datasheets and documents
References (7)
ab30930 has been referenced in 7 publications.
- Gittings LM et al. Heterogeneous nuclear ribonucleoproteins R and Q accumulate in pathological inclusions in FTLD-FUS. Acta Neuropathol Commun 7:18 (2019). PubMed: 30755280
- Chen EB et al. HnRNPR-CCNB1/CENPF axis contributes to gastric cancer proliferation and metastasis. Aging (Albany NY) 11:7473-7491 (2019). PubMed: 31527303
- Dombert B et al. Presynaptic localization of Smn and hnRNP R in axon terminals of embryonic and postnatal mouse motoneurons. PLoS One 9:e110846 (2014). PubMed: 25338097
- Glinka M et al. The heterogeneous nuclear ribonucleoprotein-R is necessary for axonal {beta}-actin mRNA translocation in spinal motor neurons. Hum Mol Genet : (2010). Zebrafish . PubMed: 20167579
- Kim C et al. Regulation of post-translational protein arginine methylation during HeLa cell cycle. Biochim Biophys Acta 1800:977-85 (2010). WB ; Human . PubMed: 20541591
- Lim Y et al. Proteomic identification and comparative analysis of asymmetrically arginine-methylated proteins in immortalized, young and senescent cells. Electrophoresis 31:3823-33 (2010). WB ; Human . PubMed: 21080485
- Hadian K et al. Identification of a heterogeneous nuclear ribonucleoprotein-recognition region in the HIV Rev protein. J Biol Chem 284:33384-91 (2009). WB ; Human . PubMed: 19808671
Images
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Western blot - Anti-hnRNP R antibody (ab30930)Anti-hnRNP R antibody (ab30930) at 1 µg/ml + HeLa Nuclear lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 71 kDaBlocking buffer: 2% BSA
Gel type: MOPS
Observed band size: 73, 77 and 80 kDa
Exposure Time: 1 minute
-
Western blot - Anti-hnRNP R antibody (ab30930)All lanes : Anti-hnRNP R antibody (ab30930) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 4 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 73,77,80 kDa why is the actual band size different from the predicted?
The three bands observed are thought to be differentially processed forms of hnRNP R. -
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP R antibody (ab30930)ICC/IF image of ab30930 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab30930, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
