Anti-PTBP2 antibody (ab228930)
Key features and details
- Rabbit polyclonal to PTBP2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PTBP2 antibody
See all PTBP2 primary antibodies -
Description
Rabbit polyclonal to PTBP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human PTBP2 (internal sequence). The exact sequence is proprietary.
Database link: Q9UKA9 -
Positive control
- IHC-P: Mouse muscle and hindbrain tissue. Rat hindbrain tissue. WB: Mouse eye tissue extracts. A549, NCI-H129 and HCT 116 whole cell lysate. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PTBP2 antibody (ab228930)All lanes : Anti-PTBP2 antibody (ab228930) at 1/2000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 57 kDa7.5% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-PTBP2 antibody (ab228930)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PTBP2 (green) using ab228930 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue), phalloidin cytoskeletal marker (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP2 antibody (ab228930)Paraffin-embedded rat hindbrain tissue stained for PTBP2 with ab228930 at a 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP2 antibody (ab228930)Paraffin-embedded mouse muscle tissue stained for PTBP2 with ab228930 at a 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP2 antibody (ab228930)Paraffin-embedded mouse hindbrain tissue stained for PTBP2 with ab228930 at a 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-PTBP2 antibody (ab228930)Anti-PTBP2 antibody (ab228930) at 1/1000 dilution + Mouse eye tissue extracts at 50 µg
Developed using the ECL technique.
Predicted band size: 57 kDa10% SDS-PAGE
