Anti-TROP2 antibody [M005] - Extracellular domain (ab253293)
![Anti-TROP2 antibody [M005] - Extracellular domain (ab253293)](https://www.abcam.com/ps/products/253/ab253293/Images/ab253293-343909-AntiTROP2-antibody-M005--Extracellular-domain-immunocytochemistry-a431--human.jpg "Anti-TROP2 antibody [M005] - Extracellular domain (ab253293)")
Key features and details
- Mouse monoclonal [M005] to TROP2 - Extracellular domain
- Suitable for: ICC/IF, WB, ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-TROP2 antibody [M005] - Extracellular domain
See all TROP2 primary antibodies -
Description
Mouse monoclonal [M005] to TROP2 - Extracellular domain -
Host species
Mouse -
Specificity
The antibody detects denatured TROP2, but with less affinity. Lower dilutions of the antibody should be used for denatured western blot.
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Tested applications
Suitable for: ICC/IF, WB, ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Other Immunogen Type within Human TROP2. The exact sequence is proprietary. Clone M005 was generated from a proprietary antigen related to the extracellular region of human TROP2 from the A431 epidermoid carcinoma cell line.
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Positive control
- WB: A431, MDA-MB-231 and MCF7 cell lysates. ICC/IF: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
M005 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody [M005] - Extracellular domain (ab253293)
Paraformaldehyde-fixed A431 (Human epidermoid carcinoma cell line) cells labeling TROP2 using ab253293 at a 1/100 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.
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![Western blot - Anti-TROP2 antibody [M005] - Extracellular domain (ab253293)](https://www.abcam.com/ps/products/253/ab253293/Images/ab253293-343906-AntiTROP2-antibody-M005--Extracellular-domain-western-blot-A431-MDAMB231-MCF7--human.jpg)
Western blot - Anti-TROP2 antibody [M005] - Extracellular domain (ab253293)All lanes : Anti-TROP2 antibody [M005] - Extracellular domain (ab253293) at 1/1000 dilution
Lane 1 : A431 (Human epidermoid carcinoma cell line) cell lysate, native conditions
Lane 2 : A431 (Human epidermoid carcinoma cell line) cell lysate, denatured conditions
Lane 3 : MDA-MB-231 (Human cell line) cell lysate, native conditions
Lane 4 : MDA-MB-231 (Human cell line) cell lysate, denatured conditions
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) cell lysate, native conditions
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) cell lysate, denatured conditions
Predicted band size: 36 kDaLanes 1, 3 & 5: Native conditions.
Lanes 2, 4 & 6: Denaturing conditions.
Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hour at room temperature.
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![ELISA - Anti-TROP2 antibody [M005] - Extracellular domain (ab253293)](https://www.abcam.com/ps/products/253/ab253293/Images/ab253293-382410-anti-trop2-antibody-m005-extracellular-domain-elisa.jpg)
ELISA - Anti-TROP2 antibody [M005] - Extracellular domain (ab253293)Representative Standard Curve using ab253293 for ELISA capture of human recombinant TROP2 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.