Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free (ab256484)
![Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free (ab256484)](https://www.abcam.com/ps/products/256/ab256484/Images/ab256484-1-ICC1anti-tim1-antibody-epr-22650-136-immunocytochemistry-huh7-human.jpg "Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free (ab256484)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22650-136] to TIM 1 - BSA and Azide free
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free
See all TIM 1 primary antibodies -
Description
Rabbit monoclonal [EPR22650-136] to TIM 1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Huh7 whole cell lysate. ICC/IF: Huh7 cells. Flow Cyt: Huh7 cells.
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General notes
Ab256484 is the carrier-free version of ab228973. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256484 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22650-136 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free (ab256484)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Huh7 (human hepatocellular carcinoma epithelial cell) cells labeling TIM 1 with ab228973 at 1/100 (5.3µg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000, 2µg/ml dilution (Green). Confocal image showing cytoplasmic punctate and membranous staining in Huh7 cell line (PMID:23233528) is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2µg/ml) dilution.
Negative control: HEK-293T（PMID: 23084921).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228973).
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![Flow Cytometry - Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free (ab256484)](https://www.abcam.com/ps/products/256/ab256484/Images/ab256484-2-FC1anti-tim1-antibody-epr22650-136-flowcytometry-huh7-human.jpg)
Flow Cytometry - Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free (ab256484)Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) / Huh7 (human hepatocellular carcinoma epithelial cell, Right) cells labeling TIM 1 with ab228973 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Negative control: HEK-293T (PMID: 23084921).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228973).
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![Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free (ab256484)](https://www.abcam.com/ps/products/256/ab256484/Images/ab256484-3-benefits-of-recombinant-antibodies.png)
Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free (ab256484)
