Canine KIM-1 ELISA Kit (ab205084)
Key features and details
- Sensitivity: 0.034 ng/ml
- Range: 0.03125 ng/ml - 1 ng/ml
- Sample type: Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine KIM-1 ELISA Kit
See all TIM 1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.034 ng/ml -
Range
0.031 ng/ml - 1 ng/ml -
Recovery
> 85 %
Sample specific recovery Sample type Average % Range Serum > 85 0.031ng/ml - 1ng/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
Kidney Injury Molecule-1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Kidney Injury Molecule-1 protein in dog biological fluids.
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Notes
In this assay the KIM-1 present in samples reacts with the anti-KIM-1 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-KIM-1, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of KIM-1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of KIM-1 in the test sample. The quantity of KIM-1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X Detection Antibody Conjugate 1 x 150µl 100X HRP Streptavidin 1 x 150µl 1X Diluent Concentrate 1 x 60ml 20X Wash Buffer Concentrate 1 x 50ml Anti-Dog KIM-1 ELISA Microplate 1 unit Chromogen Substrate Solution 1 x 12ml Dog KIM-1 Calibrator (Lyophilized) 1 vial Stop Solution 1 x 12ml -
Research areas
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Function
May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. -
Tissue specificity
Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses. -
Sequence similarities
Belongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD365
- HAVCR
- HAVCR 1
see all
Images
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Standard Curve.
Representative standard curve using ab205084 Dog KIM-1 ELISA Kit.