Anti-TIM 1 antibody [EPR22650-136] (ab228973)
![Anti-TIM 1 antibody [EPR22650-136] (ab228973)](https://www.abcam.com/ps/products/228/ab228973/Images/ab228973-352729-WB1anti-tim1-antibody-epr-22650-136-western-blot-human.jpg "Anti-TIM 1 antibody [EPR22650-136] (ab228973)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22650-136] to TIM 1
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
-
Product name
Anti-TIM 1 antibody [EPR22650-136]
See all TIM 1 primary antibodies -
Description
Rabbit monoclonal [EPR22650-136] to TIM 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Huh7 whole cell lysate. ICC/IF: Huh7 cells. Flow Cyt: Huh7 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22650-136 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-TIM 1 antibody [EPR22650-136] (ab228973)All lanes : Anti-TIM 1 antibody [EPR22650-136] (ab228973) at 1/1000 dilution
Lane 1 : Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 39 kDa
Observed band size: 140,70 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsNegative control: 293T and HeLa (PMID: 23084921).
TIM1 is often observed a band with a range of 90-140kDa due to glycosylation.
Blocking/Dilution buffer: 5% NFDM/TBST.
-
![Immunocytochemistry/ Immunofluorescence - Anti-TIM 1 antibody [EPR22650-136] (ab228973)](https://www.abcam.com/ps/products/228/ab228973/Images/ab228973-1-ICC1anti-tim1-antibody-epr-22650-136-immunocytochemistry-huh7-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TIM 1 antibody [EPR22650-136] (ab228973)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Huh7 (human hepatocellular carcinoma epithelial cell) cells labeling TIM 1 with ab228973 at 1/100 (5.3µg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2µg/ml) dilution (Green). Confocal image showing cytoplasmic punctate and membranous staining in Huh7 cell line (PMID:23233528) is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.
Negative control: HEK-293T（PMID: 23084921).
-
![Flow Cytometry - Anti-TIM 1 antibody [EPR22650-136] (ab228973)](https://www.abcam.com/ps/products/228/ab228973/Images/ab228973-2-FC1anti-tim1-antibody-epr22650-136-flowcytometry-huh7-human.jpg)
Flow Cytometry - Anti-TIM 1 antibody [EPR22650-136] (ab228973)Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) / Huh7 (human hepatocellular carcinoma epithelial cell, Right) cells labeling TIM 1 with ab228973 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Negative control: HEK-293T (PMID: 23084921).
-
![Anti-TIM 1 antibody [EPR22650-136] (ab228973)](https://www.abcam.com/ps/products/228/ab228973/Images/ab228973-4-benefits-of-recombinant-antibodies.png)
Anti-TIM 1 antibody [EPR22650-136] (ab228973)
