Anti-TIM 1 antibody [EPR17561-22] - BSA and Azide free (ab252385)
![Anti-TIM 1 antibody [EPR17561-22] - BSA and Azide free (ab252385)](https://www.abcam.com/ps/products/252/ab252385/Images/ab252385-1-252220FC1.jpg "Anti-TIM 1 antibody [EPR17561-22] - BSA and Azide free (ab252385)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17561-22] to TIM 1 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse
Overview
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Product name
Anti-TIM 1 antibody [EPR17561-22] - BSA and Azide free
See all TIM 1 primary antibodies -
Description
Rabbit monoclonal [EPR17561-22] to TIM 1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow cyt: A20 cells.
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General notes
Ab252385 is the carrier-free version of ab252220. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab252385 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17561-22 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-TIM 1 antibody [EPR17561-22] - BSA and Azide free (ab252385)
Flow cytometric analysis of Mouse splenocytes (Left panel) / A20 (mouse reticulum sarcoma cell line, Right panel) labeling TIM 1 with ab252220 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Negative control: Mouse splenocytes (PMID: 26332704; PMID: 17277130).
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252220).
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![Anti-TIM 1 antibody [EPR17561-22] - BSA and Azide free (ab252385)](https://www.abcam.com/ps/products/252/ab252385/Images/ab252385-2-benefits-of-recombinant-antibodies.png)
Anti-TIM 1 antibody [EPR17561-22] - BSA and Azide free (ab252385)
