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Immunology Adaptive Immunity T Cells Non-CD

Anti-CLEC2D antibody (ab76529)

Anti-CLEC2D antibody (ab76529)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to CLEC2D
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CLEC2D antibody
    See all CLEC2D primary antibodies
  • Description

    Rabbit polyclonal to CLEC2D
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human CLEC2D aa 100-200 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab93749)

  • Positive control

    • This antibody gave a positive signal in human spleen tissue lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human CLEC2D protein (ab132623)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Notes
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis. Inhibits osteoclast formation. Inhibits bone resorption. Modulates the release of interferon-gamma. Binds high molecular weight sulfated glycosaminoglycans.
    • Tissue specificity

      Detected in peripheral blood leukocytes, osteoblasts, lymph node, thymus and spleen. Isoform 1, isoform 2 and isoform 4 are expressed in T and B lymphocytes, and at lower levels in NK cells. They are also expressed in B-cell lines and LPS-matured monocyte-derived dendritic cells.
    • Sequence similarities

      Contains 1 C-type lectin domain.
    • Cellular localization

      Endoplasmic reticulum and Cell membrane. Cell surface.
    • Target information above from: UniProt accession Q9UHP7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 29121 Human
      • Omim: 605659 Human
      • SwissProt: Q9UHP7 Human
      • Unigene: 268326 Human
      • Alternative names

        • C type lectin related f antibody
        • C type lectin superfamily 2, member D antibody
        • C-type lectin domain family 2 member D antibody
        • CLAX antibody
        • CLC2D_HUMAN antibody
        • CLEC2D antibody
        • CLEC2D protein antibody
        • Clec2d5 antibody
        • L LT1 antibody
        • Lectin like NK cell receptor antibody
        • Lectin like transcript 1 antibody
        • Lectin-like NK cell receptor antibody
        • Lectin-like transcript 1 antibody
        • LLT-1 antibody
        • LLT1 antibody
        • OCIL antibody
        • Osteoclast inhibitory lectin antibody
        see all

      Images

      • Western blot - Anti-CLEC2D antibody (ab76529)
        Western blot - Anti-CLEC2D antibody (ab76529)
        Anti-CLEC2D antibody (ab76529) at 1 µg/ml + Human spleen tissue lysate - total protein (ab29699) at 10 µg

        Secondary
        Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 21 kDa
        Observed band size: 21 kDa
        Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.


        Exposure time: 20 minutes

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab76529? Please let us know so that we can cite the reference in this datasheet.

      ab76529 has not yet been referenced specifically in any publications.

      Images

      • Western blot - Anti-CLEC2D antibody (ab76529)
        Western blot - Anti-CLEC2D antibody (ab76529)
        Anti-CLEC2D antibody (ab76529) at 1 µg/ml + Human spleen tissue lysate - total protein (ab29699) at 10 µg

        Secondary
        Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 21 kDa
        Observed band size: 21 kDa
        Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.


        Exposure time: 20 minutes

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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