Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] - BSA and Azide free (ab223164)
![Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] - BSA and Azide free (ab223164)](https://www.abcam.com/ps/products/223/ab223164/Images/ab223164-3-ab91651280465ab91651KoWB.jpg "Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] - BSA and Azide free (ab223164)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3194] to Thymidine Kinase 1/TK1 - BSA and Azide free
- Suitable for: WB, Flow Cyt, ICC/IF
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] - BSA and Azide free
See all Thymidine Kinase 1/TK1 primary antibodies -
Description
Rabbit monoclonal [EPR3194] to Thymidine Kinase 1/TK1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HAP1 whole cell lysate. Jurkat and MOLT-4 whole cell lysate. ICC/IF: HeLa cells.
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General notes
ab223164 is the carrier-free version of ab91651 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab223164 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3194 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] - BSA and Azide free (ab223164)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: TK1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Jurkat whole cell lysate (20 µg)
Lane 4: MOLT4 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab91651 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab91651 was shown to specifically recognize TK1 in wild-type HAP1 cells along with additional cross-reactive bands. No bands were observed when TK1 knockout samples were examined. Wild-type and TK1 knockout samples were subjected to SDS-PAGE. ab91651 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab91651).
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![Immunocytochemistry/ Immunofluorescence - Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] - BSA and Azide free (ab223164)](https://www.abcam.com/ps/products/223/ab223164/Images/ab223164-1-ThymidineKinase1Primaryantibodiesab916512.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] - BSA and Azide free (ab223164) -
![Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] - BSA and Azide free (ab223164)](https://www.abcam.com/ps/products/223/ab223164/Images/ab223164-4-benefits-of-recombinant-antibodies.png)
Anti-Thymidine Kinase 1/TK1 antibody [EPR3194] - BSA and Azide free (ab223164)
