Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TFIIB antibody [EP4588] (ab109518)

Key features and details

  • Rabbit monoclonal [EP4588] to TFIIB
  • Suitable for: WB, IP, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-TFIIB antibody [EP4588]
    See all TFIIB primary antibodies

  • Description

    Rabbit monoclonal [EP4588] to TFIIB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, HeLa, Jurkat and K562 cell lysates; IHC-P: Human breast Tissue IP: Jurkat cells Flow Cyt: Jurkat

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TFIIB antibody [EP4588] (ab109518)
    Western blot - Anti-TFIIB antibody [EP4588] (ab109518)
    All lanes : Anti-TFIIB antibody [EP4588] (ab109518) at 1/1000 dilution

    Lane 1 : C6(Rat glial tumor glial cell) whole cell lysate
    Lane 2 : Raw264.7(Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 3 : PC-12(Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : NIH/3T3(Mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 35 kDa

  • Immunoprecipitation - Anti-TFIIB antibody [EP4588] (ab109518)
    Immunoprecipitation - Anti-TFIIB antibody [EP4588] (ab109518)

    ab109518 (purified) at 1/20 dilution (0.16 µg/ml) immunoprecipitating TFIIB in Jurkat whole cell lysate .
    Lane 1 (input): Jurkat(Human T cell leukemia T lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab109518 & Jurkat whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109518 in Jurkat whole cell lysate
    For western blotting, ab109518 at 1/500 and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM /TBST .

  • Flow Cytometry - Anti-TFIIB antibody [EP4588] (ab109518)
    Flow Cytometry - Anti-TFIIB antibody [EP4588] (ab109518)
    Flow cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labelling TFIIB with ab109518 at 1/20 dilution (4.05 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).
  • Western blot - Anti-TFIIB antibody [EP4588] (ab109518)
    Western blot - Anti-TFIIB antibody [EP4588] (ab109518)
    All lanes : Anti-TFIIB antibody [EP4588] (ab109518) at 1/1000 dilution

    Lane 1 : HepG2 cell lyaste
    Lane 2 : HeLa cell lyaste
    Lane 3 : Jurkat cell lyaste
    Lane 4 : K562 cell lyaste

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 35 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIB antibody [EP4588] (ab109518)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIB antibody [EP4588] (ab109518)

    ab109518, at a 1/100 dilution, staining TFIIB in paraffin embedded Human breast tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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