Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16068] to ABCF1 - C-terminal
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-ABCF1 antibody [EPR16068] - C-terminal
    See all ABCF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16068] to ABCF1 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, K562 and 293T cell lysates; Human kidney and Rat stomach tissues; HeLa and HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)
    Western blot - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)
    All lanes : Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 96 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)
    Immunocytochemistry/ Immunofluorescence - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling ABCF1 with ab190798 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue. Negative controls also shown.

  • Immunocytochemistry/ Immunofluorescence - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)
    Immunocytochemistry/ Immunofluorescence - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling ABCF1 with ab190798 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue. Negative controls also shown.

  • Western blot - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)
    Western blot - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)
    Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798) at 1/50000 dilution + 293T cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 96 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ABCF1 with ab190798 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)

    Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling ABCF1 with ab190798 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)
    Anti-ABCF1 antibody [EPR16068] - C-terminal (ab190798)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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