Anti-TFIIB (acetyl K238) antibody (ab5210)
Key features and details
- Rabbit polyclonal to TFIIB (acetyl K238)
- Suitable for: ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TFIIB (acetyl K238) antibody
See all TFIIB primary antibodies -
Description
Rabbit polyclonal to TFIIB (acetyl K238)This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide corresponding to Human TFIIB aa 200-300 (intracellular) conjugated to keyhole limpet haemocyanin.
(Peptide available asab26407)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanAll applications MouseXenopus laevisZebrafishApplication Abreviews Notes ELISA Use at an assay dependent concentration.ICC/IF Use a concentration of 5 µg/ml.Notes ELISA
Use at an assay dependent concentration.ICC/IF
Use a concentration of 5 µg/ml.Target
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Function
General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II. -
Sequence similarities
Belongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2959 Human
- Entrez Gene: 229906 Mouse
- Entrez Gene: 394390 Xenopus laevis
- Entrez Gene: 324823 Zebrafish
- Omim: 189963 Human
- SwissProt: Q00403 Human
- SwissProt: P62915 Mouse
- SwissProt: P29054 Xenopus laevis
see all -
Alternative names
- General transcription factor IIB antibody
- General transcription factor TFIIB antibody
- gtf2b antibody
see all
Images
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.-
ELISA - Anti-TFIIB (acetyl K238) antibody (ab5210)
This antibody gave a positive result in ELISA against the immunising peptide (ab26407) and a negative result using the non-modified equivalent peptide (ab25428). This indicates that it is specific for the modified peptide.
Red line: ELISA against immunising peptide ab26407
Green line: ELISA against non-modified peptide ab25428 -
Immunocytochemistry/ Immunofluorescence - Anti-TFIIB (acetyl K238) antibody (ab5210)ICC/IF image of ab5210 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab5210, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 4% PFA and 100% methanol fixed Hek293, HepG2 and Mcf7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (1)
ab5210 has been referenced in 1 publication.
- Gelev V et al. A new paradigm for transcription factor TFIIB functionality. Sci Rep 4:3664 (2014). WB ; Human . PubMed: 24441171
Images
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ELISA - Anti-TFIIB (acetyl K238) antibody (ab5210)
This antibody gave a positive result in ELISA against the immunising peptide (ab26407) and a negative result using the non-modified equivalent peptide (ab25428). This indicates that it is specific for the modified peptide.
Red line: ELISA against immunising peptide ab26407
Green line: ELISA against non-modified peptide ab25428 -
Immunocytochemistry/ Immunofluorescence - Anti-TFIIB (acetyl K238) antibody (ab5210)ICC/IF image of ab5210 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab5210, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 4% PFA and 100% methanol fixed Hek293, HepG2 and Mcf7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
