Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21954] to TATA binding protein TBP - BSA and Azide free
  • Suitable for: ChIP-sequencing, ChIP, WB, ICC/IF, IP, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free
    See all TATA binding protein TBP primary antibodies

  • Description

    Rabbit monoclonal [EPR21954] to TATA binding protein TBP - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    ChIP-seq
    Human
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293, HCT 116, MCF7, NIH/3T3 and C2C12 whole cell lysates; mouse and rat testis lysates. IP: HeLa whole cell lysate. IHC-P: Human testis and bladder cancer tissue; Mouse and rat testis tissues. ICC/IF: HeLa and HCT 116 cells. Flow cyt: HeLa cells. ChIP: Chromatin prepared from HeLa cells. ChIP-seq: Chromatin prepared from HeLa cells.

  • General notes

    Ab237785 is the carrier-free version of ab220788. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab237785 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • ChIP - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)
    ChIP - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)

    Chromatin was prepared from HeLa (human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. The ChIP was performed with 25 µg of chromatin, 5 µg of ab220788 (red), and 20 µl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (SYBR green approach).Primers and probes are located in the first kb of the transcribed region.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220788).

  • ChIP-sequencing - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)
    ChIP-sequencing - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)

    This data was developed using the same antibody clone in a different buffer formulation (ab220788).

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of Anti-TATA binding protein TBP antibody [EPR21954] - ChIP Grade (ab220788). ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed by Active Motif, Carlsbad, CA.

    Additional screenshots of mapped reads can be downloaded here.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling TATA binding protein TBP with ab220788 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining in mouse testis (PMID: 17570761; PMID: 11861477) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220788).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling TATA binding protein TBP with ab220788 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining in rat testis (PMID: 17570761; PMID: 11861477) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220788).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling TATA binding protein TBP with ab220788 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining in human bladder cancer (PMID: 17570761; PMID: 11861477) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220788).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling TATA binding protein TBP with ab220788 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining in human testis (PMID: 17570761; PMID: 11861477) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220788).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)
    Immunoprecipitation - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)

    TATA binding protein TBP was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab220788 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220788 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (Input).
    Lane 2: ab220788 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220788 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220788).

  • Flow Cytometry - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)
    Flow Cytometry - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling TATA binding protein TBP with ab220788 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220788).

  • Immunocytochemistry/ Immunofluorescence - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)
    Immunocytochemistry/ Immunofluorescence - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HCT 116 (human colorectal carcinoma cell line) cells labeling TATA binding protein TBP with ab220788 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in HCT 116 cells. The nuclear counter stain is DAPI (blue). 

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220788).

  • Immunocytochemistry/ Immunofluorescence - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)
    Immunocytochemistry/ Immunofluorescence - Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling TATA binding protein TBP with ab220788 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in HeLa cells. The nuclear counter stain is DAPI (blue). 

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220788).

  • Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)
    Anti-TATA binding protein TBP antibody [EPR21954] - BSA and Azide free (ab237785)

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