Anti-TATA binding protein TBP antibody (ab28175)
Key features and details
- Rabbit polyclonal to TATA binding protein TBP
- Suitable for: ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TATA binding protein TBP antibody
See all TATA binding protein TBP primary antibodies -
Description
Rabbit polyclonal to TATA binding protein TBP -
Host species
Rabbit -
Specificity
Ab28175 recognises TATA binding protein TBP. -
Tested Applications & Species
See all applications and species dataApplication Species ChIP Human
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Immunogen
Recombinant full length TATA binding protein TBP (Human).
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General notes
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.9
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-TATA binding protein TBP antibody (ab28175) This image is courtesy of an anonymous Abreview
ChIP analysis using ab28175 binding TATA binding protein TBP in mouse liver nuclear tissue lysate. Cells were cross-linked for 10 minutes with 1% paraformaldehyde. Samples were incubated with primary antibody for 16 hours at 4°C in TE pH8, 150mM NaCl, Trition 1%, SDS 0,1%. Protein binding was detected using real-time PCR.
Negative Control: beads. -
ChIP - Anti-TATA binding protein TBP antibody (ab28175)Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 8µg of ab28175 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).
