Antibodies, Proteins, Kits and Reagents for Life Science

381 945 ₸ Product size

100 µg 381 945 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Mouse monoclonal [1E1] to RRM2
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human
Isotype: IgG1

Product name

Anti-RRM2 antibody [1E1]
See all RRM2 primary antibodies
Description

Mouse monoclonal [1E1] to RRM2
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment, corresponding to amino acids 1-111 of Human RRM2
General notes

This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.4
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

1E1
Isotype

IgG1
Light chain type

kappa
Research areas

Western blot - Anti-RRM2 antibody [1E1] (ab57653)

Anti-RRM2 antibody [1E1] (ab57653) at 1 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Predicted band size: 45 kDa

This image was generated using the ascites version of the product.
Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody [1E1] (ab57653)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RRM2 (green) using ab57653 at 10 µg/ml in ICC/IF.

This image was generated using the ascites version of the product.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody [1E1] (ab57653)

RRM2 antibody (ab57653) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human stomach.

This image was generated using the ascites version of the product.
Western blot - Anti-RRM2 antibody [1E1] (ab57653)

RRM2 antibody (ab57653) at 1ug/lane + HeLa cell lysate at 25ug/lane.

This image was generated using the ascites version of the product.
Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody [1E1] (ab57653)

ICC/IF image of ab57653 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57653, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
Flow Cytometry - Anti-RRM2 antibody [1E1] (ab57653)

Overlay histogram showing HepG2 cells stained with ab57653 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57653, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was generated using the ascites version of the product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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