Anti-Superoxide Dismutase 1 antibody [EPR1726] - BSA and Azide free (ab185125)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1726] to Superoxide Dismutase 1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Superoxide Dismutase 1 antibody [EPR1726] - BSA and Azide free
See all Superoxide Dismutase 1 primary antibodies -
Description
Rabbit monoclonal [EPR1726] to Superoxide Dismutase 1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, MCF7, Jurkat and HT29 cell lysates; human kidney tissue.
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General notes
ab185125 is the carrier-free version of ab79390 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab185125 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1726 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Superoxide Dismutase 1 antibody [EPR1726] (ab79390) at 1/10000 dilution (purified)
Lane 1 : HeLa cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HT-29 cell lysate
Lane 5 : T-47D cell lysate
Lane 6 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?This data was developed using ab79390, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Superoxide Dismutase 1 antibody [EPR1726] (ab79390) at 1/200000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HT29 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDaThis data was developed using ab79390, the same antibody clone in a different buffer formulation.
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