Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] - BSA and Azide free (ab273095)
![Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] - BSA and Azide free (ab273095)](https://www.abcam.com/ps/products/273/ab273095/Images/ab273095-392026-antiil36gammail1f9antibodyepr23333107westernblothacat.jpg "Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] - BSA and Azide free (ab273095)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23333-107] to IL36 gamma/IL-1F9 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] - BSA and Azide free
See all IL36 gamma/IL-1F9 primary antibodies -
Description
Rabbit monoclonal [EPR23333-107] to IL36 gamma/IL-1F9 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HaCaT (treated with 20ng/ml IL-1beta and 50ng/ml TNFalpha for 20 hours,) whole cell lysate; His-tagged recombinant human IL36G protein (aa18-169).
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General notes
ab273095 is the carrier-free version of ab272731. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23333-107 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] - BSA and Azide free (ab273095)All lanes : Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731) at 1/1000 dilution
Lane 1 : HaCaT (human skin keratinocyte), whole cell lysate
Lane 2 : HaCaT treated with 20ng/ml IL-1beta and 50ng/ml TNF-alpha for 20 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 19 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression of IL36 gamma/IL-1F9 is induced by IL-1beta and TNF-alpha treatment in HaCaT (PMID: 23095752).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 81 seconds.
These data were developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab272731).
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![Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] - BSA and Azide free (ab273095)](https://www.abcam.com/ps/products/273/ab273095/Images/ab273095-2-benefits-of-recombinant-antibodies.png)
Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] - BSA and Azide free (ab273095)