Anti-S100 beta antibody - Astrocyte Marker (ab227914)
Key features and details
- Rabbit polyclonal to S100 beta - Astrocyte Marker
- Suitable for: IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-S100 beta antibody - Astrocyte Marker
See all S100 beta primary antibodies -
Description
Rabbit polyclonal to S100 beta - Astrocyte Marker -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow, Rhesus monkey -
Immunogen
Recombinant full length protein corresponding to Human S100 beta aa 1 to the C-terminus.
Database link: P04271 -
Positive control
- WB: S100 beta transfected HEK-293T whole cell extracts; A-375 whole cell extracts; Mouse and rat brain tissue extracts. IHC-P: Mouse hind brain tissue. IHC-Fr: Adult mouse retina tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Frozen sections of adult mouse retina tissue stained for S100 beta using ab227914 at 1/250 dilution in immunohistochemical analysis.
Counter stain: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody at 1/250 dilution (red).
Nuclear counter stain: Fluoroshield with DAPI (blue). -
All lanes : Anti-S100 beta antibody - Astrocyte Marker (ab227914) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with V5-human S100 beta, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 11 kDa15% SDS-PAGE gel.
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Paraffin-embedded mouse hind brain tissue stained for S100 beta using ab227914 at 1/500 dilution in immunohistochemical analysis.
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Anti-S100 beta antibody - Astrocyte Marker (ab227914) at 1/500 dilution + A-375 (human malignant melanoma cell line) whole cell extract at 30 µg
Predicted band size: 11 kDa15% SDS-PAGE gel.
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Anti-S100 beta antibody - Astrocyte Marker (ab227914) at 1/1000 dilution + Mouse brain tissue extract at 50 µg
Predicted band size: 11 kDa15% SDS-PAGE gel.
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Anti-S100 beta antibody - Astrocyte Marker (ab227914) at 1/1000 dilution + Rat brain tissue extract at 50 µg
Predicted band size: 11 kDa15% SDS-PAGE gel.