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Cancer Cell cycle Cell differentiation

Anti-S100 beta antibody - Astrocyte Marker (ab227914)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-S100 beta antibody - Astrocyte Marker (ab227914)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to S100 beta - Astrocyte Marker
  • Suitable for: IHC-P, WB, IHC-Fr
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-S100 beta antibody - Astrocyte Marker
    See all S100 beta primary antibodies
  • Description

    Rabbit polyclonal to S100 beta - Astrocyte Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Cow, Rhesus monkey
  • Immunogen

    Recombinant full length protein corresponding to Human S100 beta aa 1 to the C-terminus.
    Database link: P04271

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: S100 beta transfected HEK-293T whole cell extracts; A-375 whole cell extracts; Mouse and rat brain tissue extracts. IHC-P: Mouse hind brain tissue. IHC-Fr: Adult mouse retina tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Differentiation
    • Stem Cells
    • Neural Stem Cells
    • Glial Restricted Lineage
    • Astrocyte

Images

  • Immunohistochemistry (Frozen sections) - Anti-S100 beta antibody - Astrocyte Marker (ab227914)
    Immunohistochemistry (Frozen sections) - Anti-S100 beta antibody - Astrocyte Marker (ab227914)

    Frozen sections of adult mouse retina tissue stained for S100 beta using ab227914 at 1/250 dilution in immunohistochemical analysis.

    Counter stain: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody at 1/250 dilution (red).
    Nuclear counter stain: Fluoroshield with DAPI (blue).

  • Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914)
    Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914)
    All lanes : Anti-S100 beta antibody - Astrocyte Marker (ab227914) at 1/1000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with V5-human S100 beta, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 11 kDa



    15% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody - Astrocyte Marker (ab227914)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody - Astrocyte Marker (ab227914)

    Paraffin-embedded mouse hind brain tissue stained for S100 beta using ab227914 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914)
    Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914)
    Anti-S100 beta antibody - Astrocyte Marker (ab227914) at 1/500 dilution + A-375 (human malignant melanoma cell line) whole cell extract at 30 µg

    Predicted band size: 11 kDa



    15% SDS-PAGE gel.

  • Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914)
    Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914)
    Anti-S100 beta antibody - Astrocyte Marker (ab227914) at 1/1000 dilution + Mouse brain tissue extract at 50 µg

    Predicted band size: 11 kDa



    15% SDS-PAGE gel.

  • Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914)
    Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914)
    Anti-S100 beta antibody - Astrocyte Marker (ab227914) at 1/1000 dilution + Rat brain tissue extract at 50 µg

    Predicted band size: 11 kDa



    15% SDS-PAGE gel.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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