Detection of Human SCC-112 by Western Blot and Immunoprecipitation.
Lane 1: WB; 5 µg of NE
Lane 2: WB; 20 µg of NE
Lane 3: IP (with a different antibody) followed by WB; 1 mg of NE
Lane 4: IP (with ab17961) followed by WB; 1 mg of NE
5 to 20µg of nuclear extract (NE) from HeLa cell lysate were used for WB. 1 mg of nuclear extract (NE) from HeLa cell lysate were used for IP.
Detection: Chemiluminescence with an exposure time of less than 5 minutes.

Ab17961 was used at a dilution of  1/10,000 for the western blot and 2 µg/mg of extract for immunoprecipitation.