Human Progesterone ELISA Kit (ab108670)
Key features and details
- Sensitivity: 0.05 ng/ml
- Range: 0.2 ng/ml - 40 ng/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
-
Product name
Human Progesterone ELISA Kit
See all Progesterone kits -
Detection method
Colorimetric -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Competitive -
Sensitivity
0.05 ng/ml -
Range
0.2 ng/ml - 40 ng/ml -
Recovery
106 %
-
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Predicted to work with: Mammals
-
Product overview
Abcam’s Progesterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Progesterone in serum and plasma.
A 96-well plate has been precoated with anti-Progesterone. Samples and the Progesterone-HRP conjugate are added to the wells, where Progesterone in the sample competes with the added Progesterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Progesterone in the sample and the intensity is measured at 450 nm.
-
Platform
Microplate
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 10X Wash Solution 1 x 50ml Anti-Progesterone IgG Coated Wells 1 unit Cover foils 1 unit Progesterone control 1 x 1ml Progesterone Standard 0 - 0 ng/mL 1 x 1ml Progesterone Standard 1 - 0.2 ng/mL 1 x 1ml Progesterone Standard 2 - 1.0 ng/mL 1 x 1ml Progesterone Standard 3 - 8.0 ng/mL 1 x 1ml Progesterone Standard 4 - 40.0 ng/mL 1 x 1ml Progesterone-HRP Conjugate 1 x 22ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
Images
-
Typical Standard Curve
Representative Standard Curve using ab108670.
