Human Progesterone ELISA Kit (ab108654)
Key features and details
- Sensitivity: 0.05 ng/ml
- Range: 0.05 ng/ml - 50 ng/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Human Progesterone ELISA Kit
See all Progesterone kits -
Detection method
Colorimetric -
Sample type
Serum -
Assay type
Competitive -
Sensitivity
= 0.05 ng/ml -
Range
0.05 ng/ml - 50 ng/ml -
Recovery
111.3 %
Sample specific recovery Sample type Average % Range Serum 111.3 0.8ng/ml - 43.1ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
ab108654 is intended for the quantitative determination of progesterone concentration in Human serum.
Progesterone is a C21 steroid which is synthesized from both tissue and circulating cholesterol. Cholesterol is transformed to pregnenolone which is then converted via a combined dehydrogenase and isomerase to progesterone. The principle production sites are the adrenals and ovaries and the placenta during pregnancy. The majority of this steroid is metabolized in the liver to pregnanediol and conjugated as a glucuronide prior to excretion by the kidneys.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests Goat anti-rabbit IgG Coated Microplate (12 x 8 wells) 1 unit Progesterone Control 1 1 x 0.5ml Progesterone Control 2 1 x 0.5ml Progesterone Reference Standard 1 (0 ng/mL) 1 x 0.5ml Progesterone Reference Standard 2 (0.5 ng/mL) 1 x 0.5ml Progesterone Reference Standard 3 (3.0 ng/mL) 1 x 0.5ml Progesterone Reference Standard 4 (10 ng/mL) 1 x 0.5ml Progesterone Reference Standard 5 (25 ng/mL) 1 x 0.5ml Progesterone Reference Standard 6 (50 ng/mL) 1 x 0.5ml Progesterone-HRP Conjugate Concentrate (11x) 1 x 1.3ml Progesterone-HRP Conjugate Diluent 1 x 13ml Rabbit Anti-Progesterone Reagent (0.01%) pink color 1 x 7ml Stop Solution 1 x 11ml TMB Reagent 1 x 11ml -
Research areas