17 OH Progesterone ELISA Kit (ab108668)
Key features and details
- Sensitivity: 0.05 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
Overview
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Product name
17 OH Progesterone ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Control Sera 3 Inter-assay Sample n Mean SD CV% Control Sera 3 -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Competitive -
Sensitivity
= 0.05 ng/ml -
Recovery
98.66 %
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Assay duration
Multiple steps standard assay -
Product overview
Abcam’s 17 OH Progesterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 17 OH Progesterone in serum and plasma.
A 96-well plate has been precoated with anti-17 OH Progesterone IgG. Samples and the 17 OH Progesterone-HRP conjugate are added to the wells, where 17 OH Progesterone in the sample competes with the added 17 OH Progesterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of 17 OH Progesterone in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Solution 1 x 50ml 17 OH Progesterone Control 1 x 1ml 17 OH Progesterone Standard 0 – 0 ng/mL 1 x 1ml 17 OH Progesterone Standard 1 – 0.2 ng/mL 1 x 1ml 17 OH Progesterone Standard 2 – 0.6 ng/mL 1 x 1ml 17 OH Progesterone Standard 3 – 2.0 ng/mL 1 x 1ml 17 OH Progesterone Standard 4 – 6.0 ng/mL 1 x 1ml 17 OH Progesterone Standard 5 – 20.0 ng/mL 1 x 1ml 17 OH Progesterone-HRP Conjugate 1 x 22ml Anti-17 OH Progesterone IgG Coated Microplate (12 x 8 wells) 1 unit Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
Research areas
Images
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Typical Standard Curve
Representative Standard Curve using ab108668
