Progesterone, Endogenous progesterone receptor agonist (ab141252)
Key features and details
- Endogenous progesterone receptor agonist
- CAS Number: 57-83-0
- Purity: > 99%
- Soluble in DMSO to 100 mM and in ethanol to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Progesterone, Endogenous progesterone receptor agonist -
Description
Endogenous progesterone receptor agonist -
Biological description
Endogenous progesterone receptor agonist (EC50 = 0.5 nM) C-21 steroid hormone. Elicits a variety of biological effects.
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Purity
> 99% -
CAS Number
57-83-0 -
Chemical structure
Properties
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Chemical name
Pregn-4-ene-3,20-dione -
Molecular weight
314.47 -
Molecular formula
C21H30O2 -
PubChem identifier
12303971 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM and in ethanol to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC(=O)[C@H]2CC[C@H]3[C@@H]4CCC1=CC(=O)CC[C@]1(C)[C@H]4CC[C@]23C -
Source
Synthetic
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Research areas
Images
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Functional Studies - Progesterone, Endogenous progesterone receptor agonist (ab141252)ab16895 staining MDM2 in MCF7 cells treated with progesterone (ab141252), by ICC/IF. Decrease in MDM2 expression correlates with increased concentration of progesterone, as described in literature.
The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab141252 (progesterone) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab16895 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
