Anti-E4F1 antibody (ab70618)
Key features and details
- Rabbit polyclonal to E4F1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-E4F1 antibody
See all E4F1 primary antibodies -
Description
Rabbit polyclonal to E4F1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residue 725 and the C terminus (residue 784) of human E4F1
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Positive control
- HeLa, 293T and MCF7 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab70618 was affinity purified using an epitope specific to E4F1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-E4F1 antibody (ab70618)All lanes : Anti-E4F1 antibody (ab70618) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 84 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
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Immunoprecipitation - Anti-E4F1 antibody (ab70618)Detection of Human E4F1 by Western Blot of Immunprecipitate. Lane 1: ab70618 at 1µg/ml staining E4F1 in MCF7 whole cell lysates immunoprecipitated using ab70618 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 3 minutes.
