Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Expression system: Escherichia coli
Purity: > 98% SDS-PAGE
Endotoxin level:
Active: Yes
Suitable for: SDS-PAGE, Functional Studies

Product name

Recombinant human IL-5 protein
See all IL-5 proteins and peptides
Biological activity

The ED₅₀ as determined by the dose-dependent proliferation of TF-1 cells was ≤ 0.15 ng/ml, corresponding to a specific activity of ≥ 6.6 x 10⁶ units/mg.
Purity

> 98 % SDS-PAGE.
Sterile filtered Greater than 98% pure by SDS-PAGE and HPLC analyses.
Endotoxin level
Expression system

Escherichia coli
Accession

P05113
Protein length

Protein fragment
Animal free

No
Nature

Recombinant
- Species
  
  Human
- Sequence
  
  MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES
- Predicted molecular weight
  
  27 kDa
- Amino acids
  
  20 to 134
- Additional sequence information
  
  Disulfide-linked homodimeric protein containing two 116 amino acid chains.

Related Products
- Anti-IL-5 antibody (ab9624)

Our Abpromise guarantee covers the use of ab9625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Applications

SDS-PAGE

Functional Studies
Form

Lyophilized
Additional notes

The ED₅₀, determined by the dose-dependent stimulation of the proliferation of human TF-1 cells, is 6.6 x 10⁶ units/mg.
Concentration information loading...

Stability and Storage

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Constituent: 0.328% Sodium phosphate

This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitution

For lot specific reconstitution information please contact our Scientific Support Team.

Alternative names
- B-cell differentiation factor I
- Colony stimulating factor
- EDF
- Eosinophil differentiation factor
- IL-5
- IL5
- IL5_HUMAN
- Interleukin 5
- Interleukin 5 (colony stimulating factor, eosinophil)
- Interleukin-5
- T Cell Replacing Factor
- T-cell replacing factor
- TRF
see all
Function

Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
Sequence similarities

Belongs to the IL-5 family.
Cellular localization

Secreted.
Target information above from: UniProt accession P05113 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet

Publishing research using ab9625? Please let us know so that we can cite the reference in this datasheet.

ab9625 has not yet been referenced specifically in any publications.

Stability and Storage

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Constituent: 0.328% Sodium phosphate

This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitution

For lot specific reconstitution information please contact our Scientific Support Team.