Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant human IL-5 protein (ab9625)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 98% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant human IL-5 protein
    See all IL-5 proteins and peptides

  • Biological activity

    The ED50 as determined by the dose-dependent proliferation of TF-1 cells was ≤ 0.15 ng/ml, corresponding to a specific activity of ≥ 6.6 x 106 units/mg.

  • Purity

    > 98 % SDS-PAGE.
    Sterile filtered Greater than 98% pure by SDS-PAGE and HPLC analyses.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    P05113

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES

    • Predicted molecular weight

      27 kDa

    • Amino acids

      20 to 134

    • Additional sequence information

      Disulfide-linked homodimeric protein containing two 116 amino acid chains.

    • Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab9625 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

    • Form

      Lyophilized

    • Additional notes

      The ED50, determined by the dose-dependent stimulation of the proliferation of human TF-1 cells, is 6.6 x 106 units/mg.
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Constituent: 0.328% Sodium phosphate

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      For lot specific reconstitution information please contact our Scientific Support Team.

    General Info

    • Alternative names

      • B-cell differentiation factor I
      • Colony stimulating factor
      • EDF
      • Eosinophil differentiation factor
      • IL-5
      • IL5
      • IL5_HUMAN
      • Interleukin 5
      • Interleukin 5 (colony stimulating factor, eosinophil)
      • Interleukin-5
      • T Cell Replacing Factor
      • T-cell replacing factor
      • TRF
      see all

    • Function

      Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.

    • Sequence similarities

      Belongs to the IL-5 family.

    • Cellular localization

      Secreted.
    • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab9625? Please let us know so that we can cite the reference in this datasheet.

ab9625 has not yet been referenced specifically in any publications.

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Constituent: 0.328% Sodium phosphate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    For lot specific reconstitution information please contact our Scientific Support Team.

Images

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