Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAPGEF5 antibody [EPR6882] (ab129008)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6882] to RAPGEF5
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RAPGEF5 antibody [EPR6882]
    See all RAPGEF5 primary antibodies

  • Description

    Rabbit monoclonal [EPR6882] to RAPGEF5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human RAPGEF5 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: 293T, HeLa, and K562 cell lysates. Rat and mouse brain tissue lysates; IHC: Human testis & brain, rat cerebrum & mouse liver tissue; ICC/IF: HEK-293 and HeLa cells; Flow Cyt: HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-RAPGEF5 antibody [EPR6882] (ab129008)
    Western blot - Anti-RAPGEF5 antibody [EPR6882] (ab129008)
    All lanes : Anti-RAPGEF5 antibody [EPR6882] (ab129008) at 1/1000 dilution (Purified)

    Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cel) whole cell lysate
    Lane 4 : Mouse brain lysate
    Lane 5 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa



    Blocking/Diluting buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAPGEF5 antibody [EPR6882] (ab129008)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAPGEF5 antibody [EPR6882] (ab129008)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue sections labeling RAPGEF5 with purified ab129008 at 1/100 dilution (1.19 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-RAPGEF5 antibody [EPR6882] (ab129008)
    Western blot - Anti-RAPGEF5 antibody [EPR6882] (ab129008)
    All lanes : Anti-RAPGEF5 antibody [EPR6882] (ab129008) at 1/1000 dilution (unpurified)

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 68 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAPGEF5 antibody [EPR6882] (ab129008)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAPGEF5 antibody [EPR6882] (ab129008)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling RAPGEF5 with purified ab129008 at 1/100 dilution (1.19 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
  • Immunocytochemistry/ Immunofluorescence - Anti-RAPGEF5 antibody [EPR6882] (ab129008)
    Immunocytochemistry/ Immunofluorescence - Anti-RAPGEF5 antibody [EPR6882] (ab129008)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RAPGEF5 with Purified ab129008 at 1:500 dilution (0.24 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAPGEF5 antibody [EPR6882] (ab129008)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAPGEF5 antibody [EPR6882] (ab129008)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling RAPGEF5 with purified ab129008 at 1/100 dilution (1.19 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunocytochemistry/ Immunofluorescence - Anti-RAPGEF5 antibody [EPR6882] (ab129008)
    Immunocytochemistry/ Immunofluorescence - Anti-RAPGEF5 antibody [EPR6882] (ab129008)

    ab129008, unpurified, at 1/100 dilution, staining RAPGEF5 in HEK-293 cells by Immunofluorescence

  • Flow Cytometry - Anti-RAPGEF5 antibody [EPR6882] (ab129008)
    Flow Cytometry - Anti-RAPGEF5 antibody [EPR6882] (ab129008)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells, labeling RAPGEF5 with Purified ab129008 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor®  488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAPGEF5 antibody [EPR6882] (ab129008)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAPGEF5 antibody [EPR6882] (ab129008)

    ab129008, unpurified,  at 1/250 dilution, staining RAPGEF5 in paraffin embedded Human testis tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAPGEF5 antibody [EPR6882] (ab129008)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAPGEF5 antibody [EPR6882] (ab129008)

    ab129008, unpurified, at 1/250 dilution, staining RAPGEF5 in paraffin embedded Human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-RAPGEF5 antibody [EPR6882] (ab129008)
    OI-RD Scanning - Anti-RAPGEF5 antibody [EPR6882] (ab129008)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-RAPGEF5 antibody [EPR6882] (ab129008)
    Anti-RAPGEF5 antibody [EPR6882] (ab129008)

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