Anti-RAC2 antibody (ab2244)
Key features and details
- Goat polyclonal to RAC2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RAC2 antibody
See all RAC2 primary antibodies -
Description
Goat polyclonal to RAC2 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide:
PQPTRQQKRACSLL
, corresponding to C terminal amino acids 179-192 of Human RAC2. -
Positive control
- Recombinant Human RAC2 protein (ab78684) can be used as a positive control in WB. MOLT4 or liver lysate.
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General notes
Principal Names - RAC2; ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2); EN-7; Ras-related C3 botulinum toxin substrate 2; rho family, small GTP binding protein Rac2; Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP); Gx; HSPC022. Official Gene Symbol - RAC2. GenBank Accession Number – NP_002863. LocusLink ID - 5880.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RAC2 antibody (ab2244)All lanes : Anti-RAC2 antibody (ab2244) at 0.1 µg/ml
Lane 1 : Human thymus tissue lysate
Lane 2 : Human tonsil tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 21 kDaPrimary incubation was 1 hour. Detected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC2 antibody (ab2244)Immunohistochemical analysis of paraffin embedded Human Tonsil tissue section labelling RAC2 with ab2244 (0.3µg/ml). Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining in the interfollicular area and mantle zone but the majority of cells in germinal centre are negative. B) Higher magnification of interfollicular area showing positive cells.
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Western blot - Anti-RAC2 antibody (ab2244)Anti-RAC2 antibody (ab2244) at 0.3 µg/ml + MOLT4 cell lysate at 35 µg
Predicted band size: 21 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
