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Signal Transduction Metabolism Lipid metabolism

Anti-PTDSS1 antibody [EPR8118(2)] (ab157222)

Price and availability

264 681 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-PTDSS1 antibody [EPR8118(2)] (ab157222)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8118(2)] to PTDSS1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human, Recombinant fragment

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Overview

  • Product name

    Anti-PTDSS1 antibody [EPR8118(2)]
    See all PTDSS1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8118(2)] to PTDSS1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    corresponding to Human PTDSS1.

  • Positive control

    • Human fetal liver, Raw264.7, NIH 3T3, Human fetal brain, A549 and 293T lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8118(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Lipid metabolism

Images

  • Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222)
    Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222)
    All lanes : Anti-PTDSS1 antibody [EPR8118(2)] (ab157222) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Raw264.7 cell lysate
    Lane 3 : NIH 3T3 cell lysate
    Lane 4 : Human fetal brain lysate
    Lane 5 : A549 cell lysate
    Lane 6 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 56 kDa



    The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID: 8898108 and PMID: 10938271).

  • Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222)
    Western blot - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222)
    Anti-PTDSS1 antibody [EPR8118(2)] (ab157222) at 1/1000 dilution + His-tagged human PTDSS1 (aa2-35-GGGGS-aa2-35-GGGGS-aa2-35) recombinant protein 15ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 56 kDa


    Exposure time: 1 second


    Observed band size: 17 kDa (Recombinant protein fragment)

    Blocking buffer and concentration 5% NFDM/TBST
    Diluting buffer and concentration 5% NFDM /TBST

  • Immunoprecipitation - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222)
    Immunoprecipitation - Anti-PTDSS1 antibody [EPR8118(2)] (ab157222)

    Immunoprecipitation : 293T cell lysate immunoprecipitated with ab157222 at 1/10

    Western Blot: ab157222 at 1/1000 labeling PTDSS1 in immunoprecipitation pellet from 293T cell lysate

    Secondary Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size : 56 kDa

    The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID: 8898108 and PMID: 10938271).

  • Anti-PTDSS1 antibody [EPR8118(2)] (ab157222)
    Anti-PTDSS1 antibody [EPR8118(2)] (ab157222)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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