Anti-PTDSS1 antibody [EPR8118(2)] (ab157222)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8118(2)] to PTDSS1
- Suitable for: WB, IP
- Reacts with: Mouse, Human, Recombinant fragment
Overview
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Product name
Anti-PTDSS1 antibody [EPR8118(2)]
See all PTDSS1 primary antibodies -
Description
Rabbit monoclonal [EPR8118(2)] to PTDSS1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHumanRecombinant fragment -
Immunogen
corresponding to Human PTDSS1.
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Positive control
- Human fetal liver, Raw264.7, NIH 3T3, Human fetal brain, A549 and 293T lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8118(2) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PTDSS1 antibody [EPR8118(2)] (ab157222) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : NIH 3T3 cell lysate
Lane 4 : Human fetal brain lysate
Lane 5 : A549 cell lysate
Lane 6 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 56 kDaThe observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID: 8898108 and PMID: 10938271).
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Anti-PTDSS1 antibody [EPR8118(2)] (ab157222) at 1/1000 dilution + His-tagged human PTDSS1 (aa2-35-GGGGS-aa2-35-GGGGS-aa2-35) recombinant protein 15ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Exposure time: 1 secondObserved band size: 17 kDa (Recombinant protein fragment)
Blocking buffer and concentration 5% NFDM/TBST
Diluting buffer and concentration 5% NFDM /TBST -
Immunoprecipitation : 293T cell lysate immunoprecipitated with ab157222 at 1/10
Western Blot: ab157222 at 1/1000 labeling PTDSS1 in immunoprecipitation pellet from 293T cell lysate
Secondary Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size : 56 kDa
The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID: 8898108 and PMID: 10938271).
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