Anti-Phospholipase c eta 1 antibody (ab242092)
Key features and details
- Rabbit polyclonal to Phospholipase c eta 1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Phospholipase c eta 1 antibody
See all Phospholipase c eta 1 primary antibodies -
Description
Rabbit polyclonal to Phospholipase c eta 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human Phospholipase c eta 1 aa 1575-1625. The exact sequence is proprietary.
Database link: Q4KWH8 -
Positive control
- IP: Phospholipase c eta 1 IP in HEK-293T whole cell lysate.
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General notes
ab242092 has not performed satisfactorily when used for WB of Phospholipase c eta 1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Phospholipase c eta 1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to Phospholipase c eta 1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Phospholipase c eta 1 antibody (ab242092)
Phospholipase c eta 1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242092 at 8 µl/reaction. Western blot was performed from the immunoprecipitate using ab242092 at 1/400 dilution.
Lane 1: ab242092 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 3 minutes.
Lysates prepared in NETN buffer.
