Anti-SARS Nsp3 antibody - Coronavirus (ab181620)
Key features and details
- Rabbit polyclonal to SARS Nsp3 - Coronavirus
- Suitable for: Electron Microscopy, WB, IP, ICC/IF
- Isotype: IgG
Overview
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Product name
Anti-SARS Nsp3 antibody - Coronavirus -
Description
Rabbit polyclonal to SARS Nsp3 - Coronavirus -
Host species
Rabbit -
Specificity
This antibody is directed against SARS-Coronavirus nsp3 protein. Cross-reactivity with homologues from other sources has not been determined.
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Tested applications
Suitable for: Electron Microscopy, WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Other species -
Immunogen
This product was produced with the following immunogens:
Recombinant fragment corresponding to Human coronavirus SARS Nsp3 aa 834-943.
Sequence: VQGYKNVRITFELDERVDKVLNEKCSVYTVESGTEVTEFACVVAEAVVKT LQPVSDLLTNMGIDLDEWSVATFYLFDDAGEENFSSRMYCSFYPPDEEEE I
Database link: P0C6U8
Recombinant fragment corresponding to Human coronavirus SARS Nsp3 aa 1208-1557.
Sequence: IKACIDEVTTTLEETKFLTNKLLLFADINGKLYHDSQNMLRGEDMSFLEK DAPYMVGDVITSGDITCVVIPSKKAGGTTEMLSRALKKVPVDEYITTYPG QGCAGYTLEEAKTALKKCKSAFYVLPSEAPNAKEEILGTVSWNLREMLAH AEETRKLMPICMDVRAIMATIQRKYKGIKIQEGIVDYGVRFFFYTSKEPV ASIITKLNSLNEPLVTMPIGYVTHGFNLEEAARCMRSLKAPAVVSVSSPD AVTTYNGYLTSSSKTSEEHFVETVSLAGSYRDWSYSGQRTELGVEFLKRG DKIVYHTLESPVEFHLDGEVLSLDKLKSLLSLREVKTIKVFTTVDNTNLH
Database link: P0C6U8
Recombinant fragment corresponding to Human coronavirus SARS Nsp3 aa 1558-2040.
Sequence:TQLVDMSMTYGQQFGPTYLDGADVTKIKPHVNHEGKTFFVLPSDDTLRSE AFEYYHTLDESFLGRYMSALNHTKKWKFPQVGGLTSIKWADNNCYLSSVL LALQQLEVKFNAPALQEAYYRARAGDAANFCALILAYSNKTVGELGDVRE TMTHLLQHANLESAKRVLNVVCKHCGQKTTTLTGVEAVMYMGTLSYDNLK TGVSIPCVCGRDATQYLVQQESSFVMMSAPPAEYKLQQGTFLCANEYTGN YQCGHYTHITAKETLYRIDGAHLTKMSEYKGPVTDVFYKETSYTTTIKPV SYKLDGVTYTEIEPKLDGYYKKDNAYYTEQPIDLVPTQPLPNASFDNFKL TCSNTKFADDLNQMTGFTKPASRELSVTFFPDLNGDVVAIDYRHYSASFK KGAKLLHKPIVWHINQATTKTTFKPNTWCLRCLWSTKPVDTSNSFEVLAV EDTQGMDNLACESQQPTSEEVVENPTIQKEVIE
Database link: P0C6U8 -
Positive control
- SARS-CoV-Fr1 strain infected Vero-E6 cells.
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General notes
ab181620 was produced by repeated immunizations with 3 E.coli derived proteins representing different parts of the SARS-CoV Nsp3 protein: aa 834-943 of polyprotein 1a (ADRP domain), aa 1208-1557 of polyprotein 1a (SARS-Unique Domain) and aa 1558-2040 of polyprotein 1a (Papain-like proteinase domain).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SARS Nsp3 antibody - Coronavirus (ab181620)
PFA-fixed Vero-E6 cells stained for SARS Nsp3 (red) using ab181620 at a 1/1000 dilution in ICC/IF. Cells were infected with SARS-CoV-Fr1 strain for 1 hour at 37°C and fixed 6 hours post-infection.